Journal: Scientific Reports
Article Title: Tuning of liver circadian transcriptome rhythms by thyroid hormone state in male mice
doi: 10.1038/s41598-023-50374-z
Figure Lengend Snippet: Lowering thyroid hormone state has subtle effects on liver transcriptome rhythms. ( A ) Global DEG analysis (disregarding sampling time) is represented as a Venn diagram. ( B ) UpSet plots represent DEG analysis for each ZT separately. ( C ) Venn diagram represents all temporal DEGs (i.e., showing different expression levels of at least one ZT) identified in MMI and T 3 groups versus CON mice. ( D ) Selected examples of robust DEGs (37 in total) previously identified in T 3 -treated mice. Absolute fold change comparison of all 37 DEGs in T 3 and MMI mice are shown. Absolute fold changes were used as some genes were up- or downregulated across the groups. ( E ) Selective TH output genes and fold change of these genes. Comparisons were performed using two-way ANOVA (main treatment effect, p < 0.05). n = 3–4 for all ZTs and groups. Pair-wise comparisons were performed by Student’s t test with Welch correction. Presence (R) or absence (NR) of circadian rhythm by JTK cycle ( p value < 0.05). ***, **** represents a p value of < 0.001, and 0.0001, respectively.
Article Snippet: Data were analyzed using Transcriptome Analyses Console (Thermo Fisher Scientific, version 4.0) and expressed in log 2 values.
Techniques: Sampling, Expressing, Comparison